Guardian may refer to:
- Knuckles the Echidna, Guardian of the Master Emerald
- Guardian Units of Nations (aka G.U.N.)
- The Stone Guardian, sub-boss of the Sandopolis Zone in Sonic & Knuckles.
- The Babylon Guardian from Sonic Riders
- Lazaar's Guardian, from the SatAM episode "Super Sonic"
- The title Guardian which is any member of the Brotherhood of Guardians from the Archie Comics series
- Guardian Robots from Sonic the Comic
- Dark Guardian, a boss from Sonic Unleashed