Twinkle Snow is an ice-themedZone. The northern lights appear above the mountains in the background of this cold zone.
Minecarts offer a quick ride through portions of this Zone, along with ice machines that will suck up characters, then launch them in a frozen state (though freezing in this game is harmless). There are also platforms that the player can interact with by holding down on to make it sink slowly (and will sink faster if both characters are crouching on it), then release to be catapulted into the air. Water is also present within all three Acts of this Zone, though it is mostly avoidable by taking a high route (this also helps avoid the bottomless pits); however, Act 2 has a mandatory water section near the end. The Zone is also littered with lots of wooden platforms.
The boss of this zone is the Egg Chaser. The Egg Chaser keeps rising, so the player must use the platforms to avoid falling. Jumping off of the platforms will drop them. This is how the Egg Chaser will be damaged. Eventually, the Egg Chaser will launch a spiked ball at the player, which must be avoided. After eight hits (six in easy mode), the Egg Chaser will be defeated.